Anti-TLR4 antibody (ab13867)
Key features and details
- Rabbit polyclonal to TLR4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR4 antibody
See all TLR4 primary antibodies -
Description
Rabbit polyclonal to TLR4 -
Host species
Rabbit -
Specificity
TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to TLR4 aa 30-80.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TLR4 antibody (ab13867)All lanes : Anti-TLR4 antibody (ab13867) at 2 µg/ml
Lane 1 : Partial recombinant mouse TLR4 protein
Lane 2 : RAW cell lysate
Lane 3 : Daudi cell lysate
Lysates/proteins at 1 µg per lane.
Predicted band size: 90 kDaWestern blot analysis of TLR4 using ab13867 at 2 ug/ml. The antibody was tested against partial recombinant mouse TLR4 (extracellular portion) and a 75-80 kDa band was observed. Full length TLR4 is observed at approximately 90 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody (ab13867)Analysis of TLR4 in mouse kidney tissue using ab13867 at 5µg/ml.
