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Human SLPI ELISA Kit (ALP) (ab263890)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5.59 pg/ml
  • Range: 31.25 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human SLPI ELISA Kit (ALP)
    See all SLPI kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 6.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.6%

  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    5.59 pg/ml

  • Range

    31.25 pg/ml - 2000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 106 99% - 109%
    Serum 117 109% - 123%
    Hep Plasma 105 96% - 120%
    EDTA Plasma 119 98% - 151%
    Cit plasma 113 108% - 119%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human SLPI ELISA Kit (ALP) (ab263890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SLPI (ALP) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SLPI (ALP) with 5.59 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    SLPI (Secretory Leukocyte Protease Inhibitor), also known as ALP (Antileukoproteinase) is acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. It modulates the inflammatory and immune responses after bacterial and parasite infection.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Human SLPI standard curve comparison
    Human SLPI standard curve comparison

    Standard Curve comparison between human SLPI SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram
  • The SLPI standard curve was prepared as described in Section 10.
    The SLPI standard curve was prepared as described in Section 10.

    The SLPI standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native SLPI in human serum, plasma and urine samples.
    Interpolated concentrations of native SLPI in human serum, plasma and urine samples.

    Undiluted samples are as follows: serum 3%, plasma (citrate) 3%, plasma (EDTA) 3%, plasma (heparin) 3% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 55.8 ng/mL in neat serum, 40.9 ng/mL in neat plasma (citrate), 46.4 ng/mL in neat plasma (EDTA), 31.7 ng/mL in neat plasma (heparin) and 2.43 ng/mL in neat urine.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 62.6 ng/mL with a range of 41.2 – 85 ng/mL.

  • Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)
    Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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