Human SPARC Matched Antibody Pair Kit (ab222277)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 500 pg/ml - 32000 pg/ml
Overview
-
Product name
Human SPARC Matched Antibody Pair Kit
See all SPARC kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
160.1 pg/ml -
Range
500 pg/ml - 32000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human SPARC Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human SPARC.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab220654. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human SPARC Capture Antibody 2 x 50µg 1 x 50µg Human SPARC Detector Antibody 2 x 12.5µg 1 x 12.5µg Human SPARC Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. -
Sequence similarities
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
Developmental stage
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. - Information by UniProt
-
Alternative names
- AA517111
- Basement membrane protein 40
- Basement-membrane protein 40
see all -
Database links
- Entrez Gene: 6678 Human
- Omim: 182120 Human
- SwissProt: P09486 Human
- Unigene: 111779 Human
- Unigene: 708558 Human
Images
-
Human SPARC standard curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human SPARC Matched Antibody Pair Kit (ab222277)To learn more about the advantages of recombinant antibodies see here.
