Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.34 pg/ml
- Range: 50 pg/ml - 3200 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse LBP ELISA Kit (LPS Binding Protein)
See all LBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.5% Inter-assay Sample n Mean SD CV% Serum 3 10.1% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.34 pg/ml -
Range
50 pg/ml - 3200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84 83% - 85% Cell culture media 99 92% - 103% Hep Plasma 100 95% - 104% EDTA Plasma 97 96% - 99% Cit plasma 103 101% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LBP (LPS Binding Protein) protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LBP (LPS Binding Protein) with 8.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse LBP (LPS Binding Protein) Capture Antibody 1 x 600µl 10X Mouse LBP (LPS Binding Protein) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse LBP (LPS Binding Protein) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BPI fold containing family D, member 2
- Bpifd2
- LBP
see all -
Database links
- Entrez Gene: 16803 Mouse
- SwissProt: Q61805 Mouse
- Unigene: 218846 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse LBP standard curve in Sample Diluent NS.The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native LBP in mouse serum and plasma samples.The concentrations of LBP were measured in duplicates, interpolated from the LBP standard curves and corrected for sample dilution. Undiluted samples referred to suggested minimum dilution as follows: serum 1: 1,000, plasma (citrate) 1: 2,000, plasma (heparin) 1: 1,000, and plasma (EDTA) 1: 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 1.06 µg/mL in serum, 2.37 µg/mL in plasma (citrate), 1.53 µg/mL in plasma (heparin), and 2.12 µg/mL in plasma (EDTA).
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Sandwich ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542)To learn more about the advantages of recombinant antibodies see here.
