Human IL-21R ELISA Kit (ab277473)
Key features and details
- Sensitivity: 0.61 ng/ml
- Range: 0.614 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-21R ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.61 ng/ml -
Range
0.614 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103.8 68% - 125% Plasma 93.21 67% - 121% Cell culture media 119.5 94% - 146% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-21R ELISA Kit (ab277473) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-21R in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-21R coated on a 96-well plate. Standards and samples are pipetted into the wells and Human IL-21R present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human IL-21R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human IL-21R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human IL-21R Antibody 2 vials Anti-Human IL-21R coated Microplate 1 unit Human IL-21R standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
This is a receptor for interleukin-21. -
Tissue specificity
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsC-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD360
- IL 21R
- IL-21 receptor
see all -
Database links
- Entrez Gene: 50615 Human
- Omim: 605383 Human
- SwissProt: Q9HBE5 Human
- Unigene: 210546 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.