Anti-Pofut1 antibody (ab240479)
Key features and details
- Goat polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pofut1 antibody
See all Pofut1 primary antibodies -
Description
Goat polyclonal to Pofut1 -
Host species
Goat -
Specificity
This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human Pofut1 aa 50-150 (internal sequence) (Cysteine residue). (NP_056167.1; NP_758436.1)
Database link: Q9H488 -
Positive control
- WB: Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate. IHC-P: Human cerebral cortex tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Pofut1 antibody (ab240479)Lanes 1-2 : Anti-Pofut1 antibody (ab240479) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : Pofut1 with myc and DYKDDDDK tag transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
Lane 2 : Untransfected HE-293 cell lysate in RIPA buffer
Lane 3 : Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDaPrimary incubation for 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab240479)Paraffin-embedded human cerebral cortex tissue stained for Pofut1 using ab240479 at 3 μg/ml in immunohistochemical analysis. HRP staining.
