Anti-Pofut1 antibody (ab74302)
Key features and details
- Rabbit polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pofut1 antibody
See all Pofut1 primary antibodies -
Description
Rabbit polyclonal to Pofut1 -
Host species
Rabbit -
Specificity
ab74302 detects endogenous levels of total Pofut1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide derived from an internal region of human Pofat1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab74302)ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
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Western blot - Anti-Pofut1 antibody (ab74302)All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from HepG2 cells
Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 117 kDa, 22 kDa, 28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
