Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2000Y] to Presenilin 1/PS-1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Presenilin 1/PS-1 antibody [EP2000Y]
    See all Presenilin 1/PS-1 primary antibodies

  • Description

    Rabbit monoclonal [EP2000Y] to Presenilin 1/PS-1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Presenilin 1/PS-1 aa 300-400. The exact sequence is proprietary.
    Database link: P49768

  • Positive control

    • WB: HeLa, Mouse brain, Rat brain cell, Human fetal brain lysate. IHC-P: Human liver.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Presenilin 1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HEK293 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76083 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab76083 was shown to recognize Presenilin 1 in wild-type HAP1 cells as signal was lost at the expected MW in Presenilin 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Presenilin 1 knockout samples were subjected to SDS-PAGE. Ab76083 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Presenilin 1/PS-1 with Purified ab76083 at 1:100 dilution (1.31 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    All lanes : Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929. But we are not sure how to define the extra band around 75 KD. 

  • Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution (Purified) + Human fetal brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?



    The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929.

  • Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
    Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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