Recombinant human GSK3 beta protein (Tagged) (ab271802)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human GSK3 beta protein (Tagged)
See all GSK3 beta proteins and peptides -
Biological activity
Specific Activity: 391 pmol/min/µg.Enzyme reactions include 50 μL volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA) along with 20 μM ATP, 0.2 mg/mL GSKtide substrate, 3mM DTT, and GSTGSK3ß. After 45 min incubation at 30°C, 50 μL of the Kinase-Glo reagent solution was added and luminescence from remaining ATP was measured.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDR PQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQI MRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAK QTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCD FGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAEL LLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHP WTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKL PNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDA NTGDRGQTNNAASASASNST -
Amino acids
1 to 420 -
Tags
GST tag N-Terminus
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Preparation and Storage
- Glycogen Synthase Kinase 3 Beta
- Glycogen synthase kinase-3 beta
- GSK 3 beta
Images
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Functional Studies - Recombinant human GSK3 beta protein (Tagged) (ab271802)Specific activity of ab271802.
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SDS-PAGE - Recombinant human GSK3 beta protein (Tagged) (ab271802)SDS-PAGE analysis of 2.5 µg ab271802.