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Neuroscience Neurology process Notch Pathway

Anti-Notch2 antibody [B9] (ab245699)

Anti-Notch2 antibody [B9] (ab245699)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [B9] to Notch2
  • Suitable for: ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Notch2 antibody [B9]
    See all Notch2 primary antibodies
  • Description

    Rabbit monoclonal [B9] to Notch2
  • Host species

    Rabbit
  • Specificity

    An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2 but not NRR1.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. NRR domain of mouse Notch 2.
    Database link: O35516

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Proclin 300
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    B9
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (ab245699)
    Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (ab245699)

    PFA fixed, 0.15% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Notch2 using ab245699 at 10 µg/ml, 1 hr (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

  • Anti-Notch2 antibody [B9] (ab245699)
    Anti-Notch2 antibody [B9] (ab245699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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