Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signal that can be measured at Ex/Em = 410/450 nm when excited with a violet laser at Ex=405 nm. The dye is a hydrophobic compound that easily permeates intact live cells.

The labeling process is robust, requiring minimal hands-on time. This product can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

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