Live Cell Labeling Kit - Green Fluorescence | Cytopainter (ab187964) is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Labelling Dye Green that becomes strongly fluorescent upon entering into live cells. Labelling Dye Green is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Labelling Dye Green by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. Cells grown on black wall/clear bottom plates or slides can be stained and quantified in less than two hours at Ex/Em = 495/515 nm..

This product can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating, suspension and adherent cells.

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