Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)
![Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)](https://www.abcam.com/ps/products/251/ab251268/Images/ab251268-408154-anti-gstt1-antibody-epr19283-bsa-and-azide-free-western-blot.jpg "Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19283] to GSTT1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GSTT1 antibody [EPR19283] - BSA and Azide free
See all GSTT1 primary antibodies -
Description
Rabbit monoclonal [EPR19283] to GSTT1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251268 is the carrier-free version of ab199337 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251268 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Glutathione S Transferase theta 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19283 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab199337, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID: 24586676).
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![Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)](https://www.abcam.com/ps/products/251/ab251268/Images/ab251268-405820-anti-gstt1-antibody-epr19283-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab199337, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)](https://www.abcam.com/ps/products/251/ab251268/Images/ab251268-403486-anti-gstt1-antibody-epr19283-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab199337, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 5 seconds; Lane 4: 30 seconds.
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![Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)](https://www.abcam.com/ps/products/251/ab251268/Images/ab251268-1-anti-gstt1-antibody-epr19283-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)This data was developed using ab199337, the same antibody clone in a different buffer formulation.GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10µg (Input).
Lane 2: ab199337 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)](https://www.abcam.com/ps/products/251/ab251268/Images/ab251268-5-benefits-of-recombinant-antibodies.png)
Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (ab251268)
