Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13724] to LSECtin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LSECtin antibody [EPR13724] - BSA and Azide free
    See all LSECtin primary antibodies

  • Description

    Rabbit monoclonal [EPR13724] to LSECtin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250419 is the carrier-free version of ab181196. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250419 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    This data was developed using ab181196, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution + Mouse liver tissue lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab181196, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    This data was developed using ab181196, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    All lanes : Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 33 kDa



    This data was developed using ab181196, the same antibody clone in a different buffer formulation.

     

  • Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)
    Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

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