Human MMP13 ELISA Kit (ab221839)
Key features and details
- Sensitivity: 5.7 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow, Human, Pig
Overview
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Product name
Human MMP13 ELISA Kit
See all MMP13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 5.9% Inter-assay Sample n Mean SD CV% Supernatant 3 5% -
Sample type
Saliva, Urine, Serum, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
5.7 pg/ml -
Range
39 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 80 78% - 82% Urine 84 74% - 93% Serum 83 78% - 88% Cell culture media 102 101% - 103% -
Assay time
2h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Cow, Human, Pig -
Product overview
Human MMP13 ELISA Kit (ab221839) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP13 protein in saliva, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP13 with 5.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human MMP-13, matrix metallopeptidase 13, also known as Collagenase 3 is a secreted protein encoded by the gene MMP13. MMP-13 belongs to the matrix metalloproteinase family, which are calcium dependent endopeptidases. MMP-13 degrades type I, II, and III collagen. The MMP protein-13 is expressed as an inactive pro-form composed of an 83-amino acid pro-peptide, an amino acid catalytic domain, and hemopexin-like domain. Dissociation of a zinc ion from the cysteine-switch motif releases the activation-peptide, thus activating the enzyme. MMP-13 is involved in various processes, such as, embryonic development, ossification, wound healing, and tissue remolding.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MMP-13 Capture Antibody 1 x 600µl 10X Human MMP-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MMP-13 Lyophilized Recombinant Protein 1 x 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml Streptavidin-HRP 1 x 100µl TMB Development Solution 1 x 12ml -
Research areas
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Function
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
Tissue specificity
Seems to be specific to breast carcinomas. -
Involvement in disease
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- CLG 3
- CLG3
- Collagenase 3
see all -
Database links
- Entrez Gene: 281914 Cow
- Entrez Gene: 4322 Human
- Entrez Gene: 397346 Pig
- Omim: 600108 Human
- SwissProt: O77656 Cow
- SwissProt: P45452 Human
- Unigene: 2936 Human
Images
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Example of human MMP-13 standard curve in Sample Diluent NS
Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked MMP-13 in human serum, saliva, urine, and cell culture media samplesThe concentrations of MMP-13 were measured in duplicates, interpolated from the MMP-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, saliva 50%, urine 50%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy human donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-13 concentration was determined to be 32 pg/mL with a range of N.D. – 142 pg/mL.
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Sandwich ELISA - Human MMP13 ELISA Kit (ab221839)To learn more about the advantages of recombinant antibodies see here.
