Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4506] to Fibulin 5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free
    See all Fibulin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR4506] to Fibulin 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Fibulin 5 aa 250 to the C-terminus (internal sequence).

  • General notes

    ab247857 is the carrier-free version of ab109428 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247857 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50-60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109428, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50-60 kDa why is the actual band size different from the predicted?



    This data was developed using ab109428, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal lung lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa



    This data was developed using ab109428, the same antibody clone in a different buffer formulation.

     

  • Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
    Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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