Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
![Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)](https://www.abcam.com/ps/products/247/ab247857/Images/ab247857-406129-anti-fibulin-5-antibody-epr4506-bsa-and-azide-free-western-blot.jpg "Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4506] to Fibulin 5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free
See all Fibulin 5 primary antibodies -
Description
Rabbit monoclonal [EPR4506] to Fibulin 5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human Fibulin 5 aa 250 to the C-terminus (internal sequence).
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General notes
ab247857 is the carrier-free version of ab109428 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247857 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4506 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution
Predicted band size: 50 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?This data was developed using ab109428, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)](https://www.abcam.com/ps/products/247/ab247857/Images/ab247857-403255-anti-fibulin-5-antibody-epr4506-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?This data was developed using ab109428, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)](https://www.abcam.com/ps/products/247/ab247857/Images/ab247857-398890-anti-fibulin-5-antibody-epr4506-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (unpurified)
Lane 1 : Fetal lung lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaThis data was developed using ab109428, the same antibody clone in a different buffer formulation.
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![Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)](https://www.abcam.com/ps/products/247/ab247857/Images/ab247857-4-benefits-of-recombinant-antibodies.png)
Anti-Fibulin 5 antibody [EPR4506] - BSA and Azide free (ab247857)
