Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibulin 5 antibody [1G6A4] (ab66339)

Key features and details

  • Mouse monoclonal [1G6A4] to Fibulin 5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Fibulin 5 antibody [1G6A4]
    See all Fibulin 5 primary antibodies

  • Description

    Mouse monoclonal [1G6A4] to Fibulin 5

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant His tagged fragment, corresponding to amino acids 242-448 of Human Fibulin 5

  • Positive control

    • Colon and breast cancer

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR171104-21 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339) This image is courtesy of an anonymous Abreview
    Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µg

    Secondary
    HRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (ab66339)
    ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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