Anti-Fibulin 5 antibody [EPR4506] (ab109428)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4506] to Fibulin 5
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Fibulin 5 antibody [EPR4506]
See all Fibulin 5 primary antibodies -
Description
Rabbit monoclonal [EPR4506] to Fibulin 5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Recombinant fragment corresponding to Human Fibulin 5 aa 250 to the C-terminus (internal sequence).
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Positive control
- WB: Human fetal lung, fetal kidney, fetal spleen and testis tissue lysates. Mouse heart, brain and kidney tissue lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4506 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution
Predicted band size: 50 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (unpurified)
Lane 1 : Fetal lung lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
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