Anti-Fibulin 5 antibody (ab202977)
Key features and details
- Rabbit polyclonal to Fibulin 5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Fibulin 5 antibody
See all Fibulin 5 primary antibodies -
Description
Rabbit polyclonal to Fibulin 5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Fibulin 5 aa 126-206 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9UBX5 -
Positive control
- Human placenta tissue; Human colon carcinoma lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA
Aqueous buffered solution. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody (ab202977)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Fibulin 5 with ab202977 at 1/400 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/400 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody (ab202977)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat ovary tissue sections labeling Fibulin 5 with ab202977 at 1/500 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/500 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Western blot - Anti-Fibulin 5 antibody (ab202977)All lanes : Anti-Fibulin 5 antibody (ab202977) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Human colon carcinoma lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody (ab202977)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Fibulin 5 with ab202977 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
