Mouse VCAM1 Matched Antibody Pair Kit (ab215399)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 23.4 pg/ml - 1500 pg/ml
Overview
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Product name
Mouse VCAM1 Matched Antibody Pair Kit
See all VCAM1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.3 pg/ml -
Range
23.4 pg/ml - 1500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse VCAM1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse VCAM1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab201278. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse VCAM1 Capture Antibody 2 x 50µg 1 x 50µg Mouse VCAM1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse VCAM1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD106
- CD106 Antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
Images
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Mouse VCAM1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse VCAM1 Matched Antibody Pair Kit (ab215399)To learn more about the advantages of recombinant antibodies see here.
