Recombinant human VCAM1 protein (ab173991)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.
pH: 7.40
Constituent: 99% PBS
ab173991 was lyophilized from 0.22 µm filtered solution. 5-10% trehalose is commonly used for freeze drying and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human VCAM1 protein (ab173991)Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
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Functional Studies - Recombinant human VCAM1 protein (ab173991)Immobilized Human VCAM-1, His Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 0.01-0.156 µg/mL. -
SDS-PAGE - Recombinant human VCAM1 protein (ab173991)Reduced SDS-PAGE analysis of ab173991, stained overnight with Coomassie Blue.
Predicted MW of protein with tag is 75 kDa, but DTT-reduced protein migrates as 110-130 kDa due to glycosylation.
