Recombinant Human VCAM1 protein (ab151357)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.20
Constituents: 94% Phosphate Buffer, 0.02% Calcium chloride, 5% Trehalose, 0.02% Magnesium chloride, 0.88% Sodium chloride -
ReconstitutionAlways centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at
General Info
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Alternative names
- CD106
- CD106 Antigen
- INCAM 100
see all -
Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human VCAM1 protein (ab151357)
SDS-PAGE analysis of Recombinant Human VCAM1 protein (ab151357) under reducing conditions. VCAM1 detects a band of approximately 100 kDa due to multiple sites of glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab151357 has not yet been referenced specifically in any publications.
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Images
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SDS-PAGE - Recombinant Human VCAM1 protein (ab151357)
SDS-PAGE analysis of Recombinant Human VCAM1 protein (ab151357) under reducing conditions. VCAM1 detects a band of approximately 100 kDa due to multiple sites of glycosylation.
