Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20416-124] to p53 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-p53 antibody [EPR20416-124] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR20416-124] to p53 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LLC1 and RAW 264.7 whole cell lysate. ICC/IF: LLC1 cells. Flow Cyt: LLC1 cells. IP: p53 IP in LLC1 whole cell lysate.

  • General notes

    Ab252388 is the carrier-free version of ab246223. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LLC1 (mouse lung carcinoma) cells labeling p53 with ab246223 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077, green) at a 1/1000 dilution. Confocal image showing nuclear staining in LLC1 cells. An Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as a counterstain at 1/200 dilution, shown in Red. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246223).

  • Immunoprecipitation - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)
    Immunoprecipitation - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)

    p53 was immunoprecipitated from 0.35 mg LLC1 (Mouse lung carcinoma) whole cell lysate with ab246223 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab246223 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: LLC1 whole cell lysate (Input).
    Lane 2: ab246223 IP in LLC1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246223 in LLC1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246223).

  • Flow Cytometry - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)
    Flow Cytometry - Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized, LLC1 (Mouse lung carcinoma) cell line labeling p53 with ab246223 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (Cell without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246223).
  • Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)
    Anti-p53 antibody [EPR20416-124] - BSA and Azide free (ab252388)

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