Anti-P4HA1 antibody (ab59497)
Key features and details
- Goat polyclonal to P4HA1
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P4HA1 antibody
See all P4HA1 primary antibodies -
Description
Goat polyclonal to P4HA1 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide within Human P4HA1 aa 400-500 (internal sequence) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P13674 -
Positive control
- ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-P4HA1 antibody (ab59497)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling P4HA1 with ab59497 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml.
