Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)
Key features and details
- Sensitivity: 0.9 ng/ml
- Range: 1.563 ng/ml - 50 ng/ml
- Sample type: Cell Lysate, Milk, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Heat Shock Protein 47 ELISA Kit (SERPINH1)
See all Hsp47 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 2.9% Inter-assay Sample n Mean SD CV% Overall 7.6% -
Sample type
Milk, Serum, Plasma, Tissue, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.9 ng/ml -
Range
1.563 ng/ml - 50 ng/ml -
Recovery
96 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.
A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.
Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human HSP47 Antibody 1 x 150µl Chromogen Substrate 1 x 8ml HSP47 Microplate (12 x 8 well strips) 1 unit HSP47 Standard 1 x 2 vials Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
Involvement in disease
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Alternative names
- 47 kDa heat shock protein
- 47 kDa heat shock protein precursor
- Arsenic transactivated protein 3
see all -
Database links
- Entrez Gene: 871 Human
- Omim: 600943 Human
- SwissProt: P50454 Human
- Unigene: 596449 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108861.