Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 16, 2021

Anti-DYRK1A antibody [EPR24132-61] (ab259869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24132-61] to DYRK1A
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-DYRK1A antibody [EPR24132-61]
See all DYRK1A primary antibodies
Description

Rabbit monoclonal [EPR24132-61] to DYRK1A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HeLa transfected with scrambled siRNA control, DYRK1A knockdown HeLa , 293T, NIH/3T3, PC-12, Mouse E17 brain, P1 brain and P240 brain lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24132-61
Isotype

IgG
Research areas

Western blot - Anti-DYRK1A antibody [EPR24132-61] (ab259869)

All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : DYRK1A knockdown HeLa whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 86 kDa
Observed band size: 90 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody may react with an unidentifiable protein around 30 kDa.

Exposure time: 62 seconds
Western blot - Anti-DYRK1A antibody [EPR24132-61] (ab259869)

All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure time: 70 seconds
Western blot - Anti-DYRK1A antibody [EPR24132-61] (ab259869)

All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

Lane 1 : Mouse E17 brain tissue lysate
Lane 2 : Mouse P1 brain tissue lysate
Lane 3 : Mouse P240 brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID:28167836).

Exposure time: 15 seconds
Anti-DYRK1A antibody [EPR24132-61] (ab259869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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