Anti-MEF2D antibody (ab246876)
Key features and details
- Rabbit polyclonal to MEF2D
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2D antibody
See all MEF2D primary antibodies -
Description
Rabbit polyclonal to MEF2D -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human MEF2D aa 100-300.
Database link: Q14814 -
Positive control
- IHC-P: Human skeletal muscle, kidney and placenta tissues. WB: MEF2D HEK-293T over-expression lysate. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MEF2D antibody (ab246876)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MEF2D (green) using ab246876 at 4 µg/ml in ICC/IF.
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Western blot - Anti-MEF2D antibody (ab246876)All lanes : Anti-MEF2D antibody (ab246876) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : MEF2D HEK-293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246876)Formalin-fixed, paraffin-embedded human kidney tissue stained for MEF2D with ab246876 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246876)Formalin-fixed, paraffin-embedded human placenta tissue stained for MEF2D with ab246876 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246876)Formalin-fixed, paraffin-embedded human placenta tissue stained for MEF2D with ab246876 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246876)Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for MEF2D when incubated with ab246876 at a 1/200 dilution (negative control).