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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free
    See all alpha A Crystallin/CRYAA primary antibodies
  • Description

    Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250535 is the carrier-free version of ab181866 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250535 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as alpha A Crystallin

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14125(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Immunocytochemistry - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
    Immunocytochemistry - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
    This data was developed using ab181866, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.
  • Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
    Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
    All lanes : Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866) at 1/2000 dilution

    Lane 1 : Rat eyeball
    Lane 2 : Mouse eyeball

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab181866, the same antibody clone in a different buffer formulation.

     

  • Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
    Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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