Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)
![Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)](https://www.abcam.com/ps/products/255/ab255857/Images/ab255857-4-anti-kir2dl1-kir2dl2-antibody-epr22492-79-immunoprecipitation-nk92-human.jpg "Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22492-79] to KIR2DL1 + KIR2DL2 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22492-79] to KIR2DL1 + KIR2DL2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human KIR2DL1 recombinant protein; His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221). IHC-P: Human endometrium tissue. ICC/IF: NK-92 (treated with 5-aza-2-deoxycytidine (2µM) for 48h) cells. Flow Cyt: Human PBMCs. IP: NK-92 (treated with 5-aza-2-deoxycytidine (2µM) for 48h) whole cell lysate.
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General notes
Ab255857 is the carrier-free version of ab252937. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255857 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22492-79 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)
KIR2DL1 + KIR2DL2 were immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) (treated with 2μM 5-aza-2-deoxycytidine for 48h) whole cell lysate with ab252937 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252937 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: NK-92 (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab252937 IP in NK-92 (treated as above) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252937 IP.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252937).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)](https://www.abcam.com/ps/products/255/ab255857/Images/ab255857-3-anti-kir2dl1-kir2dl2-antibody-epr22492-79-immunohistochemistry-endometrium-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)mmunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 + KIR2DL2 with a252937 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252937).
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![Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)](https://www.abcam.com/ps/products/255/ab255857/Images/ab255857-2-anti-kir2dl1-kir2dl2-antibody-epr22492-79-immunocytochemistry-nk92-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells (+/- treatment with 5-aza-2-deoxycytidine (2μM) for 48h) labeling KIR2DL1 + KIR2DL2 with ab252937 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine (2μM) for 48h. The nuclear counte stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252937).
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![Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)](https://www.abcam.com/ps/products/255/ab255857/Images/ab255857-1-anti-kir2dl1-kir2dl2-antibody-epr22492-79-flowcytometry-pbmc-human.jpg)
Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling KIR2DL1 + KIR2DL2 with ab252937 at 1/50 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab252937 (Right). Then stained with anti-CD56 conjugated to BV421.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252937).
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![Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)](https://www.abcam.com/ps/products/255/ab255857/Images/ab255857-5-benefits-of-recombinant-antibodies.png)
Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-79] - BSA and Azide free (ab255857)
