Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6457] to Eph receptor B1/NET
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
Overview
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Product name
Anti-Eph receptor B1/NET antibody [EPR6457]
See all Eph receptor B1/NET primary antibodies -
Description
Rabbit monoclonal [EPR6457] to Eph receptor B1/NET -
Host species
Rabbit -
Specificity
Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human Eph receptor B1/NET aa 550-650 (phospho Y594). The exact sequence is proprietary.
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Positive control
- MDA-MB-435, U-87MG, NIH 3T3, COS1 and Human fetal brain cell lysates.
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General notes
Product previously known as Anti-Eph receptor B1 antibody [EPR6457].
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.13 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6457 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : U-87MG cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : COS1 cell lysate
Lane 5 : Human fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
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