Recombinant cynomolgus monkey CD86 protein (Active) (ab201434)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 97% PBS, 3% Trehalose
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is about 3-5%. Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant cynomolgus monkey CD86 protein (ab201434)
Immobilized ab201434 at 5 µg/mL (100 µL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.02-0.625 µg/mL.
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SDS-PAGE - Recombinant Cynomolgus monkey CD86 protein (ab201434)The purity of ab201434 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 50-80 kDa in SDS-PAGE due to glycosylation.
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Functional Studies - Recombinant cynomolgus monkey CD86 protein (ab201434)The purity of Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) was more than 95% as determined by SEC-HPLC. -
Functional Studies - Recombinant cynomolgus monkey CD86 protein (ab201434)Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag at 5 µg/mL (100 µL/well) can bind ab201434 with a linear range of 0.312-5 µg/mL.
