Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD45RA antibody [4KB5] (ab755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4KB5] to CD45RA
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD45RA antibody [4KB5]
    See all CD45RA primary antibodies

  • Description

    Mouse monoclonal [4KB5] to CD45RA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to CD45RA.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)
    Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Anti-CD45RA antibody [4KB5] (ab755)
    Anti-CD45RA antibody [4KB5] (ab755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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