Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [4KB5] to CD45RA
Suitable for: IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CD45RA antibody [4KB5]
See all CD45RA primary antibodies
Description

Mouse monoclonal [4KB5] to CD45RA
Host species

Mouse
Tested Applications & Species

Application Species
Flow Cyt
Human

IHC-P
Human

See all applications and species data
Immunogen

Tissue, cells or virus corresponding to CD45RA.

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

4KB5
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Anti-CD45RA antibody [4KB5] (ab755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com