Anti-HMGB1 antibody [ABM24D3] (ab208282)
![Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252286-anti-hmgb1-antibody-abm24d3-western-blot.jpg "Anti-HMGB1 antibody [ABM24D3] (ab208282)")
Key features and details
- Mouse monoclonal [ABM24D3] to HMGB1
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HMGB1 antibody [ABM24D3]
See all HMGB1 primary antibodies -
Description
Mouse monoclonal [ABM24D3] to HMGB1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human HMGB1 aa 1-200.
Database link: P09429 -
Positive control
- HepG2 lysate; Human adenocarcinoma of stomach, Human prostate, Human squamous cell carcinoma of esophagus and Human spleen tissues; PBMC and Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM24D3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HMGB1 antibody [ABM24D3] (ab208282)Anti-HMGB1 antibody [ABM24D3] (ab208282) at 2 µg/ml + HepG2 lysate
Predicted band size: 25 kDa
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![Flow Cytometry - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252292-anti-hmgb1-antibody-abm24d3-flow-cytometry.jpg)
Flow Cytometry - Anti-HMGB1 antibody [ABM24D3] (ab208282)Flow Cytometrical analysis of Jurkat cells using ab208282 at 0.5 µg/106 cells (Red). Green represents isotype control. Goat anti-mouse PE conjugate was used as secondary antibody.
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![Flow Cytometry - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252291-anti-hmgb1-antibody-abm24d3-flow-cytometry.jpg)
Flow Cytometry - Anti-HMGB1 antibody [ABM24D3] (ab208282)Flow Cytometrical analysis of PBMC (lymphocyte) cells using ab208282 at 0.5 µg/106 cells (Red). Green represents isotype control. Goat anti-mouse PE conjugate was used as secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252290-anti-hmgb1-antibody-abm24d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling HMGB1 with ab208282 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252289-anti-hmgb1-antibody-abm24d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human squamous cell carcinoma of esophagus tissue labeling HMGB1 with ab208282 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252288-anti-hmgb1-antibody-abm24d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling HMGB1 with ab208282 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)](https://www.abcam.com/ps/products/208/ab208282/Images/ab208282-252287-anti-hmgb1-antibody-abm24d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody [ABM24D3] (ab208282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of stomach tissue labeling HMGB1 with ab208282 at 1 µg/ml.
