Anti-EPS15R antibody [EP1147Y] (ab53006)
![Anti-EPS15R antibody [EP1147Y] (ab53006)](https://www.abcam.com/ps/products/53/ab53006/Images/ab53006-65598-anti-eps15r-antibody-ep1147y-western-blot.jpg "Anti-EPS15R antibody [EP1147Y] (ab53006)")
Key features and details
- Rabbit monoclonal [EP1147Y] to EPS15R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EPS15R antibody [EP1147Y]
See all EPS15R primary antibodies -
Description
Rabbit monoclonal [EP1147Y] to EPS15R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EPS15R aa 800-900 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1147Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EPS15R antibody [EP1147Y] (ab53006)Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15R antibody [EP1147Y] (ab53006)](https://www.abcam.com/ps/products/53/ab53006/Images/ab53006-65597-anti-eps15r-antibody-ep1147y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15R antibody [EP1147Y] (ab53006)ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
