Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free
    See all Cytokeratin 5 primary antibodies

  • Description

    Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431 cells. Human transitional urinary bladder carcinoma.

  • General notes

    ab214586 is the carrier-free version of ab52635 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab214586 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586) Image from Khanom R. et al PLoS One. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163. eCollection 2016.

    Colocalization of KRT5, KRT6 and KRT17 in HSC3 cells

    Immunocytochemistry in HSC3 (human oral squamous carcinoma cell line) cells. Scale bar, 10 μm.

    (Taken from Figure S3 of Khanom et al)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Clone EP1601Y (ab214586) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 647). Please refer to ab193895 for protocol details.

    ab193895 staining Cytokeratin 5 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab193895 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in A431 cells fixed with 100% methanol (5min).

  • Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Clone EP1601Y (ab214586) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 488). Please refer to ab193894 for protocol details.

    ab193894 staining Cytokeratin 5 in HACAT cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab193894 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Flow Cytometry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Clone EP1601Y (ab214586) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 5 antibody [EP1601Y] (PE). Please refer to ab224985 for protocol details.

    Overlay histogram showing A431 cells stained with ab224985 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224985, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 µg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Flow Cytometry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Flow Cytometry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunocytochemistry - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    A431 cells stained with unpurified ab52635 at 1/100 - 1/250

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    This IHC data was generated using the same anti-Cytokeratin 5 antibody clone, EP1601Y, in a different buffer formulation (cat# ab52635).

    ab52635 showing positive staining in squamous cell cervical carcinoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

    This IHC data was generated using the same anti-Cytokeratin 5 antibody clone, EP1601Y, in a different buffer formulation (cat# ab52635).

    ab52635 showing positive staining in basal cell breast carcinoma tissue.

  • Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
    Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)

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