Anti-Cytokeratin 17 antibody [Ks17.E3] (ab19067)
![Anti-Cytokeratin 17 antibody [Ks17.E3] (ab19067)](https://www.abcam.com/ps/products/19/ab19067/Images/ab19067-28593-anti-cytokeratin-17-antibody-ks17e3-immunofluorescence.jpg "Anti-Cytokeratin 17 antibody [Ks17.E3] (ab19067)")
Key features and details
- Mouse monoclonal [Ks17.E3] to Cytokeratin 17
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Cytokeratin 17 antibody [Ks17.E3]
See all Cytokeratin 17 primary antibodies -
Description
Mouse monoclonal [Ks17.E3] to Cytokeratin 17 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Cytokeratin 17 (Human).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Ks17.E3 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [Ks17.E3] (ab19067)ICC/IF image of ab19067 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19067, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
