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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)

Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5804] to eIF3B - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-eIF3B antibody [EPR5804] - BSA and Azide free
    See all eIF3B primary antibodies
  • Description

    Rabbit monoclonal [EPR5804] to eIF3B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells. ICC/IF: HeLa cells
  • General notes

    Ab238921 is the carrier-free version of ab133601. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes. 

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238921 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 5.60 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5804
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133601).

  • OI-RD Scanning - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
    OI-RD Scanning - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133601).

  • Flow Cytometry - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
    Flow Cytometry - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)

    Flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133601).

  • Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)
    Anti-eIF3B antibody [EPR5804] - BSA and Azide free (ab238921)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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