Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF3B antibody [EPR5804] (ab133601)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5804] to eIF3B
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-eIF3B antibody [EPR5804]
    See all eIF3B primary antibodies

  • Description

    Rabbit monoclonal [EPR5804] to eIF3B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human eIF3B aa 150-250. The exact sequence is proprietary.

  • Positive control

    • A431, 293T, Jurkat, HeLa and Caco 2 cell lysate; HeLa cells ICC/IF: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.60 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5804

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-eIF3B antibody [EPR5804] (ab133601)
    Western blot - Anti-eIF3B antibody [EPR5804] (ab133601)
    All lanes : Anti-eIF3B antibody [EPR5804] (ab133601) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 92 kDa
    Observed band size: 116 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (ab133601)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (ab133601)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Flow Cytometry - Anti-eIF3B antibody [EPR5804] (ab133601)
    Flow Cytometry - Anti-eIF3B antibody [EPR5804] (ab133601)
    Flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).
  • OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (ab133601)
    OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (ab133601)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF3B antibody [EPR5804] (ab133601)
    Anti-eIF3B antibody [EPR5804] (ab133601)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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