Recombinant Human Betatrophin protein (ab203551)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, SDS-PAGE, MS
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
0.05 M acetate buffer
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ReconstitutionAdd 0.1 M acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Images
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SDS-PAGE - Recombinant Human Betatrophin protein (ab203551)
14% SDS-PAGE separation of ab203551.
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and heated sample, 2.5 μg / lane
3. Non-reduced and non-heated sample, 2.5 μg / lane