Anti-Calponin 2 antibody (ab233204)
Key features and details
- Rabbit polyclonal to Calponin 2
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Calponin 2 antibody
See all Calponin 2 primary antibodies -
Description
Rabbit polyclonal to Calponin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Cow, Orangutan
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Calponin 2 aa 1-300. EXpressed in E.coli.
Database link: Q99439 -
Positive control
- WB: Recombinant human Calponin 2 protein; Human lung lysate; Pig lymph node lysate; HeLa and HepG2 cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calponin 2 antibody (ab233204)Anti-Calponin 2 antibody (ab233204) at 2 µg/ml + Recombinant human Calponin 2 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 34 kDa
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Western blot - Anti-Calponin 2 antibody (ab233204)All lanes : Anti-Calponin 2 antibody (ab233204) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Pig lymph node lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 34 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Calponin 2 antibody (ab233204)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calponin 2 (green) using ab233204 at 20 μg/ml in ICC/IF. FITC labeling.
