Anti-eIF3B antibody (ab264230)
Key features and details
- Rabbit polyclonal to eIF3B
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF3B antibody
See all eIF3B primary antibodies -
Description
Rabbit polyclonal to eIF3B -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human eIF3B aa 100-150. The exact sequence is proprietary.
Database link: P55884 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates; IP: HeLa whole cell lysate; IHC-P Human ovarian carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF3B antibody (ab264230)All lanes : Anti-eIF3B antibody (ab264230) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.Detected with chemilimuniscence with exposure time of 10 seconds.
4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody (ab264230)Immunohistochemical analysis of FFPE human ovarian carcinoma tissue section labeling eIF3B with ab264230 at 1/200 dilution. Detection: DAB.
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Immunoprecipitation - Anti-eIF3B antibody (ab264230)eIF3B was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264230 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264230 at 1 µg/ml.
Lane 1: ab264230 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
