Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR17078] to Cytokeratin 7
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Cytokeratin 7 antibody [EPR17078]
    See all Cytokeratin 7 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR17078] to Cytokeratin 7

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment within Mouse Cytokeratin 7 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9DCV7

  • Positive control

    • WB: Mouse Lung tissue lysate; HeLa and A549 whole cell lysate IHC-P: normal human breast tissue sections

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17078

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    Western blot - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    All lanes : HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945) at 1/5000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : KRT7 knockout A549 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 51 kDa



    ab209945 was shown to specifically react with KRT7 in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab209945 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/20000 dilution for 1 hour at room temperature. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)

    IHC image of Cytokeratin 7 staining in a section of formalin-fixed paraffin-embedded normal human breast*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209945, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    Western blot - HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    All lanes : HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945) at 1/5000 dilution

    Lane 1 : Lung (Mouse) Tissue Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209945 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)
    HRP Anti-Cytokeratin 7 antibody [EPR17078] (ab209945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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