Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP27] to Cytokeratin 5 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free
    See all Cytokeratin 5 primary antibodies

  • Description

    Rabbit monoclonal [SP27] to Cytokeratin 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytokeratin 5 (C terminal). The exact sequence is proprietary.
    Database link: P13647

  • Positive control

    • WB: HepG2 whole cell lysate (ab166833), A431 whole cell lysate. IHC-P: Human prostate adenocarcinoma, prostate, thymus, skin, pancreatic adenocarcinoma, lung, lung squamous cell carcinoma, breast, cervical squamous cell carcinoma, bladder and bladder transtitional cell carcinoma tissue. Mouse skin and Rat skin tissues. IHC-Fr: Mouse and rat skin tissue section.Flow Cyt: A431 cells. ICC/IF: A431 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab236216 is the carrier-free version of ab64081. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236216 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer
    tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human prostate cancer, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64081 for 30 mins at room temperature. This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 5 with purified ab64081 at 1/30 (8.2 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab64081 at 1/100 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 5 with purified ab64081 at 1/30 (8.2 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Flow Cytometry - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling Cytokeratin 5 with ab64081 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on rat skin, performed on a Leica Biosystems BOND™ RX instrument.

    The section was incubated with ab64081 for 30 mins at room temperature.

    This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse skin, performed on a Leica Biosystems BOND™ RX instrument
    The section was incubated with ab64081 for 30 mins at room temperature. This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung squamous cell cancer tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on tumor cells of human lung squamous cell cancer, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64081 for 30 mins at room temperature. This image was generated using ab64081, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64081).

     

  • Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)
    Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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