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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)

Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [10C11E6] to Cytokeratin 5
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Cytokeratin 5 antibody [10C11E6]
    See all Cytokeratin 5 primary antibodies
  • Description

    Mouse monoclonal [10C11E6] to Cytokeratin 5
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment corresponding to Human Cytokeratin 5 aa 316-590. (Expressed in E.coli).
    Sequence:

    THVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKY EELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIA DAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDV EIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGG LGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSA SSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS


    Database link: P13647
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Cytokeratin 5 recombinant protein; A431 cell lysate; Human bladder cancer and esophagus cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    10C11E6
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Images

  • Western blot - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Western blot - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Anti-Cytokeratin 5 antibody [10C11E6] (ab181491) at 1/500 dilution + A431 cell lysate

    Predicted band size: 62 kDa

  • Western blot - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Western blot - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Anti-Cytokeratin 5 antibody [10C11E6] (ab181491) at 1/500 dilution + Human Cytokeratin 5 recombinant protein.

    Predicted band size: 62 kDa



    Expected MWt is 47.8 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)

    Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling Cytokeratin 5 with ab181491 at 1/200 dilution, with DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [10C11E6] (ab181491)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Cytokeratin 5 with ab181491 at 1/200 dilution, with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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