Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PKC delta (phospho Y311) antibody [EPR2609Y]
    See all PKC delta primary antibodies

  • Description

    Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)

  • Host species

    Rabbit

  • Specificity

    ab76181 only detects PKC delta phosphorylated on Tyrosine 311.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic phospho-peptide from residues surrounding Tyrosine 311 of human PKC delta.

  • Positive control

    • WB: HeLa cell lysate treated with Phorbol-12-myristate-13-acetate and U937 cell lysate treated with TPA. IHC-P: Human urinary bladder carcinoma tissue. IP: HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/500 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

    Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

    Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with ab76181 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

    ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

    Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
    Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

    For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP)  at a dilution of 1/1000.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/5000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : U937 cell lysate treated with TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit-HRP at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?

  • Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
    Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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