Anti-PKC delta (phospho S299) antibody [EPNCI119] - BSA and Azide free (ab188170)
![Anti-PKC delta (phospho S299) antibody [EPNCI119] - BSA and Azide free (ab188170)](https://www.abcam.com/ps/products/188/ab188170/Images/ab188170-326123-anti-pkc-delta-phospho-s299-antibody-epnci119-immunohistochemistry.jpg "Anti-PKC delta (phospho S299) antibody [EPNCI119] - BSA and Azide free (ab188170)")
Key features and details
- Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299) - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC delta (phospho S299) antibody [EPNCI119] - BSA and Azide free
See all PKC delta primary antibodies -
Description
Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7 cell lysate treated with Caylculin A; Human transitional cell carcinoma tissue.
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General notes
ab188170 is the carrier-free version of ab133456 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab188170 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
The auto-phosphorylation of PKC delta at pS299 targeted by Ab133456 was identified in the following paper: FEBS Lett. 2007 Jul 24;581(18):3377-81. Novel phosphorylation site markers of protein kinase C delta activation. Durgan J1, Michael N, Totty N, Parker PJ.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPNCI119 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho S299) antibody [EPNCI119] - BSA and Azide free (ab188170)
Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma tissue labelling PKC delta phospho S299 with ab133456 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133456).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
