Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1483(N)] to PKC (phospho S729) - BSA and Azide free
Suitable for: Dot blot, WB
Reacts with: Rat, Human

Product name

Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free
See all PKC primary antibodies
Description

Rabbit monoclonal [EPR1483(N)] to PKC (phospho S729) - BSA and Azide free
Host species

Rabbit
Specificity

This antibody cross-reacts with several isoforms of PKC.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab250360 is the carrier-free version of ab181119. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250360 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR1483(N)
Isotype

IgG
Research areas

Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/2000 dilution

Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate.
Lane 2 : THP-1 whole cell lysate treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 24 hours.
Lane 3 : THP-1 whole cell lysate treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 24 hours. Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 84 kDa
Additional bands at: 84 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 3 seconds

This data was developed using ab181119, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/1000 dilution

Lane 1 : Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag at 0.02 µg
Lane 2 : Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag at 0.06 µg
Lane 3 : Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag at 0.02 µg
Lane 4 : Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag at 0.06 µg
Lane 5 : Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag at 0.02 µg
Lane 6 : Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag at 0.02 µg
Lane 7 : Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag at 0.02 µg
Lane 8 : Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag at 0.02 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 84 kDa
Additional bands at: 100-150 kDa. We are unsure as to the identity of these extra bands.

This data was developed using ab181119, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1, 3, 5-8: 10 seconds; Lanes 2, 4: 3 minutes.
Dot Blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

This data was developed using ab181119, the same antibody clone in a different buffer formulation.Dot blot analysis of PKC epsilon (pS729) phospho peptide (Lane 1), PKC epsilon non-phospho peptide (Lane 2), labelling PKC (pS729) with purified ab181119 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/2000 dilution

Lane 1 : Lysate of SH-SY5Y cells treated with Okadaic Acid and Calyculin A
Lane 2 : Lysate of SH-SY5Y cells

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 84 kDa

This data was developed using ab181119, the same antibody clone in a different buffer formulation.
Anti-PKC (phospho S729) antibody [EPR1483(N)] - BSA and Azide free (ab250360)

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