Anti-PKC delta (phospho T505) antibody (ab60992)
Key features and details
- Rabbit polyclonal to PKC delta (phospho T505)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC delta (phospho T505) antibody
See all PKC delta primary antibodies -
Description
Rabbit polyclonal to PKC delta (phospho T505) -
Host species
Rabbit -
Specificity
Detects endogenous levels of PKC delta only when phosphorylated at threonine 505. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human PKC delta (internal sequence).
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Positive control
- WB: Extracts from NIH/3T3cells, treated with UV (15mins); C57BL/6 Mouse splenocytes cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC delta (phospho T505) antibody (ab60992)All lanes : Anti-PKC delta (phospho T505) antibody (ab60992) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3cells,
treated with UV (15mins).
Lane 2 : Extracts from NIH/3T3cells,
treated with UV (15mins) with immunizing phosphopeptide.
Predicted band size: 77 kDa
Observed band size: 77 kDa
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Western blot - Anti-PKC delta (phospho T505) antibody (ab60992)Anti-PKC delta (phospho T505) antibody (ab60992) at 1/500 dilution + C57BL/6 Mouse splenocytes cells at 40 µg
Secondary
HRP Anti-rabbit IgG Goat (H&L) at 1/1000 dilution
Predicted band size: 77 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho T505) antibody (ab60992)ab60992 staining human normal tonsil tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
