Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [PKCgT514-PF4] to PKC (phospho T514)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC (phospho T514) antibody [PKCgT514-PF4]
    See all PKC primary antibodies

  • Description

    Rabbit monoclonal [PKCgT514-PF4] to PKC (phospho T514)

  • Host species

    Rabbit

  • Specificity

    Pan-specific for PKC. Recognises gamma Thr514.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PKC (phospho T514). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P17252

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: NIH/3T3 (treated with TPA) cell extract; C6 cell extract. Flow cyt: Jurkat cells; HT1080 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    PKCgT514-PF4

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

    Flow cytometric analysis of Jurkat cells, treated with K252a and stained with the secondary antibody only negative control (blue) or treated with K252a (red) or untreated (green) using ab278787 at 0.1 µg/mL. Cat. #2451.

  • Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    All lanes : Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787) at 0.1 µg/ml

    Lane 1 : C6 cell extract (treated with staurosporine)
    Lane 2 : C6 cell extract (untreated)
  • Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

    Flow cytometric analysis of HT1080 cells treated with staurosporine (red) or untreated (green) usingab278787 at 0.05 ug/mL,  or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with staurosporine (black) or untreated (blue). 

  • Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Peptide blocking flow cytometric analysis of HT1080 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated with staurosporine and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated with staurosporine and blocked with non-phospho peptide (purple) using ab278787 at 0.05 µg/mL.
  • Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    All lanes : Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787) at 0.05 µg/ml

    Lane 1 : Mouse embryo fibroblast cell line (NIH/3T3) cell extract (untreated)
    Lane 2 : Mouse embryo fibroblast cell line (NIH/3T3) cell extract (treated with TPA)
  • Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)
    Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

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