Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [PKCgT514-PF4] to PKC (phospho T514)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PKC (phospho T514) antibody [PKCgT514-PF4]
See all PKC primary antibodies
Description

Rabbit monoclonal [PKCgT514-PF4] to PKC (phospho T514)
Host species

Rabbit
Specificity

Pan-specific for PKC. Recognises gamma Thr514.
Tested Applications & Species

Application Species
Flow Cyt
Mouse

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human PKC (phospho T514). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P17252

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Positive control
- WB: NIH/3T3 (treated with TPA) cell extract; C6 cell extract. Flow cyt: Jurkat cells; HT1080 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Mouse
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

PKCgT514-PF4
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

Flow cytometric analysis of Jurkat cells, treated with K252a and stained with the secondary antibody only negative control (blue) or treated with K252a (red) or untreated (green) using ab278787 at 0.1 µg/mL. Cat. #2451.
Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

All lanes : Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787) at 0.1 µg/ml

Lane 1 : C6 cell extract (treated with staurosporine)
Lane 2 : C6 cell extract (untreated)
Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

Flow cytometric analysis of HT1080 cells treated with staurosporine (red) or untreated (green) usingab278787 at 0.05 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with staurosporine (black) or untreated (blue).
Flow Cytometry - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

Peptide blocking flow cytometric analysis of HT1080 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated with staurosporine and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated with staurosporine and blocked with non-phospho peptide (purple) using ab278787 at 0.05 µg/mL.
Western blot - Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

All lanes : Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787) at 0.05 µg/ml

Lane 1 : Mouse embryo fibroblast cell line (NIH/3T3) cell extract (untreated)
Lane 2 : Mouse embryo fibroblast cell line (NIH/3T3) cell extract (treated with TPA)
Anti-PKC (phospho T514) antibody [PKCgT514-PF4] (ab278787)

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