5,7-Dichlorokynurenic acid, NMDA receptor glycine site antagonist (ab120023)
Key features and details
- NMDA receptor glycine site antagonist
- CAS Number: 131123-76-7
- Purity: > 99%
- Soluble in 1 eq. NaOH to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
5,7-Dichlorokynurenic acid, NMDA receptor glycine site antagonist -
Description
NMDA receptor glycine site antagonist -
Biological description
Potent NMDA receptor glycine site antagonist. Water soluble form available - see (ab120254).
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Purity
> 99% -
CAS Number
131123-76-7 -
Chemical structure
Properties
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Chemical name
5,7-Dichloro-4-hydroxyquinoline-2-carboxylic acid -
Molecular weight
258.06 -
Molecular formula
C10H5Cl2NO3 -
PubChem identifier
1779 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in 1 eq. NaOH to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O)c1cc(O)c2c(Cl)cc(Cl)cc2n1 -
Source
Synthetic
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Research areas
Images
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Functional Studies - 5,7-Dichlorokynurenic acid, NMDA receptor glycine site antagonist (ab120023)ab12416 staining cGMP in SKNSH cells treated with 5,7-Dichlorokynurenic acid (ab120023), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 5,7-Dichlorokynurenic acid, as described in literature.
The cells were incubated at 37°C for 20 minutes in media containing different concentrations of ab120023 (5,7-Dichlorokynurenic acid) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.